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KMID : 0363620140350030060
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Journal of Korean Oriental Medicine
2014 Volume.35 No. 3 p.60 ~ p.69
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Comparative Study on Biological Activities of Gwakhyangjeonggi-san Decoction According to the Preservation Periods
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Jin Seong-Eun
Kim Ohn-Soon
Shin Hyeun-Kyoo
Jeong Soo-Jin
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Abstract
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Background: Herbal formulas are generally served as a type of decoction. However, there is no scientific evidence for determining preservation and circulation period of herbal medicine decoctions. Thus, we investigated anti-inflammatory and anti-oxidative effects of the Gwakhyangjeonggi-san decoction according to its preservation period.
Methods: Gwakhyangjeonggi-san decoction was stored for 0, 1, 2 or 3 months at room temperature. To evaluate anti-inflammatory effects, enzyme-linked immunosorbent assays (ELISAs) for tumor necrosis factor-alpha (TNF-) and interleukin-6 (IL-6), and nitric oxide (NO) assay were conducted using the culture supernatant from RAW 264.7 cells stimulated with lipopolysaccharide (LPS). The antioxidant activities were studied by measuring free radical scavenging activities on ABTS and DPPH.
Results: Gwakhyangjeonggi-san decoction maintained the inhibitory effects on TNF-, IL-6 and NO productions after up to 2 months of storage in LPS-treated RAW 264.7 cells. No inflammatory response was observed in 3 months of storage. In addition, the scavenging activities on ABTS and DPPH of Gwakhyangjeonggi-san decoction were reduced time-dependently and showed less than 50% inhibition after 3 months of storage.
Conclusions: Our results suggest that preservation period of Gwakhyangjeonggi-san decoction is recommended within 2 months after storage.
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KEYWORD
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Gwakhyangjeonggi-san decoction, preservation period, anti-inflammation, anti-oxidation
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